Protein Identification

The goal is to determine which proteins are present in your sample. The sample can be a cellular extract, a gel spot or band, a purified protein, etc.

Briefly, the sample is digested with trypsin after reduction of disulfide bonds and alkylation of free thiol groups. The digestion can be performed in solution or directly in gel.

The resulting peptides are analyzed either by LC-MS/MS (most of the samples) or by MALDI-TOF/TOF (mostly 2D gel spots, or isolated pure proteins). Once data recorded, they are submitted to database search using Mascot or SEQUEST HT search engine in order to identify proteins. Amino acid sequences from the organism of origin have therefore to be available in public databases like SwissProt, NCBI, etc. or should be provided to be inserted in an in-house confidential database.

Learn more about Protein Identification of Proteins Mixture (Including 1D Gel Band)

Learn more about High Throughput Protein Identification from 2D Gels (>48 Spots) by MALDI-TOF/TOF