Protein Sequencing and Extensive Characterization

As primary sequence plays an essential role in structural characterization of proteins, it is important to have tools allowing providing a complete or very high sequence coverage. Generally, this cannot be achieved by traditional trypsin digestion because large parts of proteins are not detected or efficiently sequenced.

We propose here two strategies to overcome this limitation and provide very high sequence coverages: one digestion multi-enzymatic in solution (MELD) and a N-terminal and C-terminal sequencing method using MALDI-ISD.

To fully characterize a protein, attention to detection and localization of post-translational modifications is mandatory. MELD and other technics can help studying PTMs of a purified protein.

Learn more about Optimized Sequencing using Multi-Enzymatic-Limited-Digestion (MELD)

Learn more about Top-Down Sequencing

Learn more about PTMs Characterization