Protein Identification of Proteins Mixture (Including 1D Gel Band)

This is the method of choice for identifying a protein with high confidence starting from a solution or a gel.

Depending on the sample complexity, several analysis parameters will be adapted (length of chromatographic separation, use of 2D-LC, mass spectrometer used) and therefore influence the price. The price includes the trypsin digestion, the LC-MS/MS analysis, the database search and the reporting (pdf format). Please contact us for a personalized quote.

What is delivered?

A list of proteins identified with relevant statistical values. For these proteins, you will know which peptides were identified.

Report and results will be sent by email. Results are generally available in 15 working days.

Raw data are available on request.

What is needed for these analyses?

For in solution digestion, at least 10 µg of proteins are required. Please note that if the buffer is not compatible with the workflow, an additional purification step (see Sample Preparation Section for more details) will be performed

For in gel digestion, the bands/spots must be visible by Coomassie blue staining to be identified.

- Tip for 1D-PAGE: please provide us the entire gel and a scan showing which bands have to be processed. The picking will be performed manually at the facility.

Analysis Request Form for Identification of Protein(s) by LC-ESI-MS/MS