Top-Down Sequencing

The protein is directly deposited on a MALDI plate with the appropriate matrix, inducing the fragmentation of the protein during the laser shot. Mass spectrum displays peaks allowing determining parts of the sequence. By this technique, N- and C- termini can be sequenced over maximum 60 AA on both sides. This technics is therefore more recommended for peptides and small proteins. For top down sequencing of larger proteins, please contact us.

What is delivered?

A list of sequence tags will be provided. For known sequences, a database search can be performed. In this case, proteins identified with relevant statistical values will be provided.

Report and results will be sent by email. Results are generally available in 15 working days.

Raw data are available on request.

What is needed for these analyses?

At least 30 µg of a purified protein are required (minimum concentration of 50 µM). Please note that if the buffer is not compatible with the workflow or the solution is too diluted, an additional purification/concentration step (see Sample Preparation Section for more details) will be performed. If you have less material, please contact us to discuss the feasibility of your demand.

If you know your protein and want to confirm its sequence or a single amino acid mutation:

Analysis Request Form for Top-Down Sequencing by MALDI-ISD

If you want to obtain N-term or C-term tags de novo:

Analysis Request Form for de novo Sequencing by MALDI-TOF/TOF