PTMs Characterization
Most of PTMs and chemical modifications are neither labile nor heterogeneous and can therefore be localized during the database search step. Please provide us information concerning potential PTMs and/or modifications present in your samples when ordering a protein identification service.
However, some PTMs are trickier to study and need more complex sample preparation:
- N-Glycosylation
This PTM is one of the most difficult to study because of its natural heterogeneity. We propose you to characterize it at two levels:
- The glycopeptides level: LC-MS/MS data are searched for specific signature of glycopeptides allowing glycans to be localized and partially characterized.
- The glycans level: glycans are released thanks to enzymatic process and are analyzed by mass spectrometry. A list of all detected glycans is generated but no information about their localization is obtained. This can be performed on complex mixtures such as serum.
Please contact us for further information and feasibility.
- For other PTMs (Disulfide bonds, Phosphorylation, others): please contact us for further information and feasibility.
