Label-free differential proteomics: Relative quantification
The principle of this strategy is to compare in a quantitative fashion, tryptic peptides digest of complex samples and spiked with different commercial internal standards which allows their relative quantification. The high sensitivity, resolution and mass accuracy of the mass spectrometer allow confident identification of the peptides and proteins present in the samples to be compared.
The generated LC-MS/MS data are submitted to database search using open-source software MaxQuant. After this step of identification and normalization, the data can be compared and submitted to statistical analysis using open-source software Perseus.
More information on these softwares
Please note that for the study to be statistically relevant, each condition to be compared should be represented by at least biological triplicates.
Contact us for details on experimental procedure, results delivery time and personalized quotation.
Analysis Request Form for Label-free Differential Proteomic Analysis
